Figure 3.

Effect of KIAA0247 expression on colony formation, proliferation, and cell cycle progression of non‐small‐cell lung cancer (NSCLC) cells. A549 and H1299 cells were transfected with KIAA0247‐specific siRNA (si‐KIAA0247) or control siRNA, or with a KIAA0247 expression plasmid (p‐KIAA0247) or vehicle (control). *P < .05 and **P < .01. A, Colony formation assay. (i) A549: si‐KIAA0247 vs control, P = .002; p‐KIAA0247 vs control, P = .04. (ii) H2199: si‐KIAA0247 vs control, P = .039; p‐KIAA0247 vs control, P = .02. B, MTT assay. (i) A549: si‐KIAA0247 vs control, P = .001; p‐KIAA0247 vs P = .002. (ii) H1299: si‐KIAA0247 vs control, P = .05; p‐KIAA0247 vs control, P = .001. C, Cell cycle analysis. KIAA0247 overexpression results in blockage of the cell cycle at the G1/S transition. D,E, Changes in expression of cell proliferation‐related proteins in (D) A549 and (E) H1299 cells