Figure 4.

CD44s expression is associated with a mesenchymal phenotype and migratory ability in hepatocellular carcinoma (HCC) cells. A, Highly metastatic cell lines have higher migration potential than less metastatic cell lines. B, Expression of CD44s, E‐cadherin, N‐cadherin and Snail was detected by western blotting in HCC cell lines. C, Expression levels of CD44s, E‐cadherin and N‐cadherin were detected by western blotting in CD44s‐overexpressing PLC/PRF/5 cells. D, Cell migration was measured using transwell assays in CD44s‐overexpressing PLC/PRF/5 cells. E, Expression levels of CD44s, E‐cadherin and N‐cadherin were detected by western blotting in CD44s‐knockdown MHCC‐97L cells. F, Cell migration was measured using transwell assays in CD44s‐knockdown MHCC‐97L cells. **P < .01