Figure 6.

Tunicamycin (TM) inhibits hyaluronic acid (HA)‐induced cell migration by suppressing ERK1/2 activity in MHCC‐97L cells. A, Expression levels of p‐ERK1/2 and ERK1/2 were detected by western blotting in MHCC‐97L cells treated with 50 μg/mL HA for the indicated length of time. B, Expression levels of p‐ERK1/2 and ERK1/2 were detected by western blotting in MHCC‐97L cells treated with 2.5 μg/mL TM, 50 μg/mL HA or 10 μmol/L U0126 alone or in combination for 12 h. C, MHCC‐97L cells were treated with 2.5 μg/mL TM, 50 μg/mL HA or 10 μmol/L U0126 alone or in combination for 12 h, and migration was measured using transwell assays. *P < .05