Figure 7.

Tunicamycin (TM) inhibits CD44 expression and alters its glycosylation. A, MHCC‐97L and MHCC‐97H cell lysates were treated with PNGase F, Endo Hf, or α‐2,3‐neuraminidase at 37°C according to the datasheets. Shift in CD44 molecular weight was detected by western blotting. B, Expression level of CD44s was detected by western blotting in SMMC‐7721 and MHCC‐97L cells treated with 2.5 μg/mL TM for 24 h. C, Expression of CD44s was detected by real‐time PCR in MHCC‐97L, MHCC‐97H and SMMC‐7721 cells treated with 2.5 μg/mL TM for 24 h. *P < .05; **P < .01