Figure 2.

FBW7 overexpression inhibited the malignant biological behaviors of glioblastoma multiforme (GBM). A, The efficiency of FBW7 overexpression (OE) and shRNA lentivirus vector in U251 and U373 cell lines were detected by western blot assay. B, The cell viability of U251 and U373 was assessed by MTT assay in non‐transfected cells, cells transfected with eGFP lentivirus, cells stably overexpressing FBW7 and cells stably transfected with FBW7‐specific shRNA. C‐E, Representative photographs of invasion cells in both U251 and U373 cell lines by transwell assay. Scale bars = 100 μm. Data of invasive cell numbers from 3 independent experiments are presented in the form of histograms. F‐I, Representative fluorescent pictures with red strip to show the result of wound healing assay in E and F. Scale bars = 100 μm. Data of migrated cell numbers of different groups were analyzed in the form of line charts. (*P < .05, **P < .01, ***P < .001 compared to blank control)