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. 2018 Feb 12;19(4):e44810. doi: 10.15252/embr.201744810

Figure EV4. Time‐lapse imaging of GFP‐TrkB trafficking in primary cortical neurons from WT and S1/3 KO mice.

Figure EV4

  1. Representative kymographs (time–space plots) generated from time‐lapse movies visualizing trafficking of transiently overexpressed GFP‐TrkB in DIV7 primary cortical neurons from WT and S1/3 KO mice.
  2. Segmental velocity of GFP‐TrkB vesicles uninterruptedly moving in retrograde or anterograde direction in axons from WT and S1/3 KO primary cortical neurons. (n = 15–20 neurons/genotype from two neuronal culture preparations each).
  3. Population (% of total) of GFP‐TrkB vesicles moving in retrograde and anterograde directions in axons from WT and S1/3 KO primary cortical neurons (n = 28–29 neurons/genotype from three neuronal culture preparations each).
  4. Total distance covered by GFP‐TrkB vesicles uninterruptedly moving in retrograde or anterograde directions in axons from WT and S1/3 KO primary cortical neurons. (n = 15–20 neurons/genotype from two neuronal culture preparations each).
Data information: Data in (B–D) are shown as mean ± SEM and analyzed using two‐way ANOVA with Bonferroni post‐test.