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. 2018 Feb 1;19(4):e44993. doi: 10.15252/embr.201744993

Figure EV5. ZBTB2 acts as a gene activator and stimulates cellular differentiation (related to Fig 5).

Figure EV5

  1. Percentages of expressed and not expressed genes in the total transcriptome of wild‐type ESCs and the subset of ZBTB2‐bound genes (*P = 8.348e‐09, Fisher's exact test).
  2. RNA‐seq analysis of gene expression levels in Zbtb2 KO ESCs, relative to wild‐type ESCs. Data are depicted as mean of two biological replicates.
  3. Western blot analyses of protein levels in wild‐type and Zbtb2 KO ESCs. Histone H3 was used as a loading control.
  4. qRT–PCR analysis of gene expression levels in wild‐type, Zbtb2 knockout (KO) and Zbtb2 KO ESCs transiently transfected with ZBTB2 [Zbtb2 rescue (RC)]. Data are depicted as mean ± SEM of three biological replicates (*P < 0.05 compared to WT, Welch t‐test).

Source data are available online for this figure.