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. 2018 Feb 28;19(4):e45484. doi: 10.15252/embr.201745484

Figure EV5. HP1 promotes centromeric localization of Haspin to antagonize Wapl (related to Fig 5).

Figure EV5

  • A, B
    Example images of cells described in Fig 5A and C.
  • C, D
    HeLa and the indicated HP1 DKO clones were treated with nocodazole for 3 h. Mitotic chromosome spreads were immunostained. Example images are shown (C). The centromeric Sgo1/CENP‐C immunofluorescence intensity ratio was determined on around 100 chromosomes in 10 cells (D) (unpaired t‐test).
  • E, F
    Chromosome spreads prepared from nocodazole‐arrested mitotic HeLa and HP1 DKO clone 3A2 cells transiently expressing Myc‐SA2‐K290E were immunostained. Then, the inter‐KT distance was measured on over 400 chromosomes in over 20 cells (unpaired t‐test) (E), and lysates of asynchronous cells were immunoblotted (F).
Data information: Means and SDs are shown (D and E). Scale bars, 10 μm.Source data are available online for this figure.