Table 2.

Characterization of active site motifs and domains identified in gene 6513 coding for β-fructofuranosidase (FFase)

Domain				Pfam		Predicted binding site residues using different tools			Active site residues predicted by I-Tasser
NCBI-CDD	214,757	185,737	185,718	PF00251	PF08244	TM-SITE	S-SITE	COACH
Motif	Glycosyl hydrolases family 32	Glycosyl hydrolase family 32, beta-fructosidases	Glycosyl hydrolase families: GH43, GH62, GH32, GH68	Glycosyl hydrolases family 32 N-terminal	Glycosyl hydrolases family 32 C-terminal
Gene 6513	Position 46–526	Position 52–397	Position 62–395	Position 46–340	Position 468–667	Ligands (fructose, sucrose, nystose) G55, D56, L74, T78, F114, D115, R186, D187, E264, T265, Y337	Ligands (glucose, kestose, sucrose) D56, L74, F114, D115, L137, P138, I139, H140, R186, E264, E289, S298, S300, Y337, W365, F371, G372	Ligands (fructose) G55, D56, L74, T78, F114, D115, R186, D187, E264, Y337, W365	D56 and E264