Fig. 2.

Treatment of persister cells with different combinations. a The difference in survivability of persister cells after 18 h of treatment with a combination of ampicillin with different carbon sources (50 mM glucose, 50 mM glycerol, and 25 mM mannitol). The maximum reduction in the viability was recorded in mannitol plus ampicillin combination. Asterisks indicate the statistically significant difference (p < 0.01) in different treatment combinations as compare to control of persister cells treated with ampicillin alone (80 μg/ml). b %survival rate after 18 h of treatment with different combinations of nisin, ampicillin with mannitol. The maximum reduction in the viability was recorded in combination C. Asterisks indicate the statistically significant difference (p < 0.01) in different treatment combinations as compare to control of untreated persister cells. c Cells were stained with fluorescein isothiocyanate (FITC, final concentrations 420 nM) after 2 h of treatment; a shift in fluorescence peak was recorded that shows dead population. c(i) control of persister cells, c(ii) Shift was recorded showing dead population after treatment with combination containing 100 µg/ml Nisin + 0.5 µg/ml Ampicillin + 25 mM Mannitol, c(iii) Comparatively more shift was recorded after treatment with combination containing 200 µg/mlNisin + 1.0 µg/ml Ampicillin + 25 mM Mannitol than c(ii) combination