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. 2018 Apr 3;9:297. doi: 10.3389/fphar.2018.00297

FIGURE 1.

FIGURE 1

D-dencichine treatment increases the number of circulating platelets in mice with thrombocytopenia induced by carboplatin. (A) The chemical structure of D-dencichine. (B) Experimental schedule of D-dencichine administration in Balb/c mice. The six group mice were received NS or IL-11 or D-dencichine for 7 days. On the 7th day, the other five groups then intravenously injected with carboplatin (80 mg/kg), whereas the control group was intravenously injected with volumetric NS. After that, each group was treated with NS or IL-11 or D-dencichine for another 7 days. (C) Peripheral platelet counts under the administration of IL-11 (0.37 mg/kg) or D-dencichine (0.75, 1.50, and 3.0 mg/kg) in mice for different time intervals (0, 7, and 14 days) subjected to thrombocytopenia induced by carboplatin (on day 7), (n = 10). (D) Reticulated platelet count was determined by flow cytometry. (E) Quantification of the percentage of platelets in vivo biotinylated platelets 24, 48, 72, and 168 h after sulfo-NHS-biotin injection (1 h after injection; n = 5). ##P < 0.01 vs. control group; ∗P < 0.05, ∗∗P < 0.01 vs. model group.