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1H NMR spectra of native GBS type V and mutant GBSVsia2,6 capsular polysaccharides (CPSs). (A) Wild-type GBS type V, D2O, 500 MHz, 30° pulse, 80°C. (B) GBSVsia2,6 (Δcps5K/cps2N) mutant, 33 mM phosphate pD 8.0 in D2O, 700 MHz, 30° pulse, 61°C.
