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. 2018 Apr 3;9:545. doi: 10.3389/fmicb.2018.00545

Figure 5.

Figure 5

Capsular polysaccharide (CPS) expression levels and sialic acid linkage in S. suis serotype 2 and 14 mutants carrying exogenous α-2,3-sialyltransferase. (A) Hydrophobicity (%) of the wild-type S. suis serotype 2 (SS2) and 14 (SS14) strains, the SS2sia2,3 (Δcps2N/cpsK) and SS14sia2,3 (Δcps14N/cpsK) mutants carrying the GBS α-2,3-sialyltransferase (cpsK). The non-encapsulated mutants SS2Δcps and SS14Δcps were used as control strains. (B,C) Whole-bacterial cell enzyme-linked lectin assay (ELLA) was performed to detect α-2,3 or α-2,6 capsular sialic acid linkage in SS2sia2,3 and SS14sia2,3 mutant strains. Whole bacteria were incubated with Sambucus nigra agglutinin (SNA-I) specific for Neu5Ac α-2,6 linkages, or Maackia amurensis leukoagglutinin (MAL-I) specific for Neu5Ac α-2,3 linkages. The non-encapsulated mutants SS2Δcps and SS14Δcps were used as negative controls. SS2 was used as positive control for SNA-I and wild-type GBS type III as positive control for MAL-I. Data in (A–C) are expressed as mean ± SEM of at least three independent experiments. Student's t-test analyses reported significant differences between “a” and “b” (P < 0.05).