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Transmission electron micrographs showing capsular polysaccharide (CPS) expression by GBS type V isogenic mutants. The CPS was labeled with polycationic ferritin. GBS type V wild-type strain was surrounded by a consistent CPS layer, whereas the GBSVΔsiaT (Δcps5K), the GBSVΔsynth (Δneu5B), and the GBSVsia2,6 (Δcps5K/cps2N) mutants all showed intermediate state of encapsulation. The non-encapsulated mutant strain GBSVΔcps is depicted as negative control. Bars = 1 μm.
