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. 2018 Apr 3;9:545. doi: 10.3389/fmicb.2018.00545

Figure 9.

Figure 9

H NMR spectra of native GBS type V and sialic acid-deficient mutant capsular polysaccharides (CPSs). (A) Wild-type, native CPS, D2O, 500 MHz, 30° pulse, 80°C. (B) GBSVΔsynth (Δneu5B) mutant, native CPS, D2O, 300 MHz, 90° pulse, 60°C. (C) GBSVΔsiaT (Δcps5K) mutant, native CPS, 33 mM phosphate pD 8.0 in D2O, 500 MHz, 90° pulse with presaturation, 67°C. (D) Wild-type, chemically desialylated polysaccharide, D2O, 500 MHz, 30° pulse, 80°C.