Table 1.
Bacterial strains and plasmids used in this study.
| Strain/Plasmid | General Characteristics | Source/References |
|---|---|---|
| Escherichia coli | ||
| TOP 10 | F-mrcA Δ(mrr-hsdRMS-mcrBC)φ80 lacZΔM5 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| MC1061 | araD139 Δ(ara-leu)7697 ΔlacX74 galU galK hsdR2(rK-mK+) mcrB1 rpsL | Casadaban and Cohen, 1980 |
| Streptococcus suis (SS) serotype 2 | ||
| SS2 WT | P1/7 wild type (WT), highly encapsulated serotype 2 strain isolated from a clinical case of swine infection in the United Kingdom | Slater et al., 2003 |
| SS2Δcps | Non-encapsulated isogenic mutant strain derived from strain P1/7. In-frame deletion of the cps2F gene (rhamnosyltransferase; locus tag: SSU0520) | Lecours et al., 2011a |
| SS2ΔsiaT | Isogenic mutant strain derived from strain P1/7. Indirect in-frame deletion of the cps2N gene (sialyltransferase; locus tag: SSU0533) | This work |
| SS2compΔsiaT | Mutant SS2ΔsiaT complemented with pMX1cps2N | This work |
| SS2sia2,3 | Isogenic mutant strain derived from strain P1/7. Indirect substitution of the cps2N gene by the cps3K gene of GBS type III (sialyltransferase; locus tag: GBSCOH1_1070) | This work |
| SS2Δsynth | Non-encapsulated isogenic mutant strain derived from strain P1/7. In-frame deletion of the neu2C gene (UDP-N-acetylglucosamine 2-epimerase; locus tag: SSU0536) | Lecours et al., 2011a |
| SS2Δsynth/ΔsiaT | Isogenic mutant strain derived from strain P1/7. In-frame deletions of the neu2C and cps2N genes | This work |
| SS2Δsynth/sia2,3 | Isogenic mutant strain derived from strain P1/7. In-frame deletion of the neu2C gene and substitution of the cps2N gene by the cps3K gene of GBS type III | This work |
| Streptococcus suis (SS) serotype 14 | ||
| SS14 WT | DAN13730 wild type (WT), highly encapsulated serotype 14 strain isolated from a human case in the Netherlands | Gottschalk et al., 1989 |
| SS14Δcps | Non-encapsulated isogenic mutant strain derived from strain DAN13730. In-frame deletion of the cps14B gene (chain length determinant protein Wzd; locus tag: equivalent of SSU0516 in P1/7) | Roy et al., 2015 |
| SS14sia2,3 | Isogenic mutant strain derived from strain DAN13730. Indirect substitution of the sialyltransferase cps14N gene (locus tag: equivalent of SSU0533 in P1/7) by the cps3K gene of GBS type III | This work |
| SS14Δsynth | Non-encapsulated isogenic mutant strain derived from strain DAN13730. In-frame deletion of the neu14C gene (UDP-N-acetylglucosamine 2-epimerase; locus tag: equivalent of SSU0536 in P1/7) | Roy et al., 2015 |
| SS14Δsynth/sia2,3 | Isogenic mutant strain derived from strain DAN13730. In-frame deletion of the neu14C gene and substitution of the cps14N gene by the cps3K gene of GBS type III | This work |
| Group B Streptococcus (GBS) serotype III | ||
| GBSIII WT | COH1 wild type (WT), well-encapsulated type III strain isolated from an infant with sepsis and meningitis | Chaffin et al., 2005 |
| GBSIIIsia2,6 | Isogenic mutant strain derived from strain COH1. Direct substitution of sialyltransferase cps3K gene by cps2N gene of S. suis type 2 | This work |
| GBSIIIΔcps | Non-encapsulated isogenic mutant strain derived from strain COH1. In-frame deletion of the cpsE gene (glucosyltransferase; locus tag: GBSCOH1_1075) | Lecours et al., 2012 |
| GBSIIIΔsynth | Intermediate-encapsulated isogenic mutant strain derived from strain COH1. In-frame deletion of the neu3B gene (sialic acid synthase; locus tag: GBSCOH1_1066) | Lecours et al., 2012 |
| Group B Streptococcus (GBS) serotype V | ||
| GBSV WT | CJB111 wild type (WT), well-encapsulated type V strain isolated from neonate with septicemia | ATCC |
| GBSVsia2,6 | Isogenic mutant strain derived from strain CJB111. Direct substitution of cps5K gene (sialyltransferase; locus tag: SAG1163) by cps2N gene of S. suis type 2 | This work |
| GBSVΔsynth | Isogenic mutant strain derived from strain CJB111. In-frame deletion of the neu5B gene (sialic acid synthase; locus tag: SAG1161) | This work |
| GBSVΔsiaT | Isogenic mutant strain derived from strain CJB111. In-frame deletion of the cps5K gene | This work |
| GBSVΔcps | Non-encapsulated isogenic mutant strain derived from strain CJB111. In-frame deletion of the cpsE gene (glucosyltransferase; locus tag: SAG1171) | Lemire et al., 2014 |
| Plasmids | ||
| pCR2.1 | Apr, Kmr, oriR(f1) MCS oriR (ColE1) | Invitrogen |
| pSET4s | Thermosensitive vector for allelic replacement. Replication functions of pG+host3, MCS oriR pUC19 lacZ SpR | Takamatsu et al., 2001b |
| pMX1 | Replication functions of pSSU1, MCS pUC19 lacZ SpR, malQ promoter of S. suis, derivative of pSET2 | Takamatsu et al., 2001b |
| p4ΔneuC | pSET4s carrying the construct for neuC allelic replacement (S. suis serotypes 2 and 14) | This work |
| p4Δcps2N | pSET4s carrying the construct for cps2N indirect allelic replacement | This work |
| p4neuC | pSET4s carrying intact neuC gene for neuC reintroduction (S. suis serotypes 2 and 14) | This work |
| p4sia2,3_2 | pSET4s carrying construct for allelic replacement of S. suis cps2N gene by cps3K from GBS type III | This work |
| p4sia2,3_14 | pSET4s carrying construct for allelic replacement of S. suis cps14N gene by cps3K from GBS type III | This work |
| p4sia2,6_III | pSET-4s carrying construct for allelic replacement of GBS type III cps3K gene by cps2N from S. suis type 2 | This work |
| p4sia2,6_V | pSET4s carrying construct for allelic replacement of GBS type V cps5K gene by cps2N from S. suis type 2 | This work |
| p4Δneu5B | pSET4s carrying construct for allelic deletion of GBS type V neu5B gene | This work |
| p4Δcps5K | pSET4s carrying construct for allelic deletion of GBS type V cps5K gene | This work |
| pMX1cps2N | pMX1 complementation vector carrying intact cps2N gene | This work |