Figure 1.

Toc86 is a proteolytic fragment of a larger polypeptide. Chloroplasts were isolated from pea seedlings in the absence (−) or presence (+) of a protease inhibitor cocktail. The chloroplasts were lysed and fractionated to yield an envelope membrane fraction. Envelope membranes (25 μg of protein) were resolved by SDS-PAGE and immunoblotted with anti-Toc86 (anti-Toc86), anti-Toc75 (anti-Toc75), or anti-Toc34 (anti-Toc34) IgG. The molecular masses of known proteins are shown to the left of the figure.