Table 2.
HMGA2 immunohistochemistry and HMGA2 interphase FISH analysis
| Case no.1 | HMGA2 expression by immunohistochemistry | % nuclei with HMGA2 and 14q24 probe co-localization by interphase FISH2 |
|---|---|---|
| 1 | Positive (+4) | 823 |
| 2 | Positive (+4) | 944 |
| 3 | Positive (+4) | Not Determined5 |
| 4 | Positive (+4) | 90 |
| 5 | Positive (+4) | 906 |
| 6 | Positive (+4) | Not Determined |
| 7 | Positive (+4) | 90 |
| 8 | Negative (0) | 14 |
| 9 | Negative (0) | 13 |
| 10 | Negative (0) | 16 |
| 11 | Negative (0) | 9 |
| 12 | Negative (0) | 10 |
Cases ordered by HMGA2 immunohistochemistry expression status
When grouped by immunohistochemistry expression status, the mean percentages of FISH probe co-localization were significantly different (p=8.24×10−10): 89.2% (95% confidence interval: 83.8–94.6%) vs. 12.4% (95% confidence interval: 8.8–16.0%) for cases with positive and negative HMGA2 protein expression, respectively.
Interphase FISH showed HMGA2 amplification in 96/100 nuclei.
Interphase FISH showed that 69/100 nuclei had three hybridization signals for HMGA2, and was previously reported as ST00-142 to have der(14)t(12;14)(q15;q24) and three hybridization signals for HMGA2 in metaphase FISH (Table 1, published as ST00-142) (19).
Previously reported to have der(14)t(12;14)(q15;q24) and three hybridization signals for HMGA2 in metaphase FISH (Table 1, published as ST02-165) (18).
Interphase FISH showed that 20/100 nuclei had three hybridization signals for HMGA2.