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. Author manuscript; available in PMC: 2018 Apr 10.
Published in final edited form as: Methods Enzymol. 2018 Feb 24;601:309–330. doi: 10.1016/bs.mie.2017.11.031

Figure 6.

Figure 6

PCR (sequencing libraries) of a wild-type time course. A. Samples from different time points (0, 2, 4 and 6 h) were resolved on a 5% non-denaturing polyacrylamide gel. The first lane is a control where the non-biotinylated P5 adaptor was used. The smear at 200–700 bp (brackets) indicates PCR-amplified adaptor-ligated fragments and is excised from the gel. The DNA is extracted and ethanol precipitated prior to next-generation sequencing. B. Bioanalyzer (Agilent) lane profile showing the S1-seq library size distribution (∼150–600 bp).