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. Author manuscript; available in PMC: 2018 Apr 10.
Published in final edited form as: Methods Enzymol. 2018 Feb 24;601:309–330. doi: 10.1016/bs.mie.2017.11.031
Component Per reaction (μL) Final Concentration
Beads in 10 mM Tris-HCl 10
5× Phusion HF buffer 10 1 ×
10 mM dNTPs 1 0.2 mM
Phusion High-Fidelity 2U/μL 0.5 1 U
10 μM primer P5 2 0.4 μM
10 μM primer P7 (indexed) 2 0.4 μM
ddH2O 24.5
Total volume 50