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. 2018 Mar 17;11(3):327–331. doi: 10.14202/vetworld.2018.327-331

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Copyright: © Tahir, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-1 — The experimental design for measuring serum cortisol level in Epinephalus fuscoguttatus exposed to different salinities. Two hundred fingerlings were acclimatized in a 500 L tank with 31 ppt salinity for 2 weeks. After acclimatization, 10 fish were introduced into each tank containing 22 L seawater adjusted at different salinities of 31 ppt, 25 ppt, 20 ppt, 15 ppt, 10 ppt and 5 ppt with triplicates. Blood sampling was done after 5, 30, 60 and 120 min. followed by observation for survival percentage for two weeks.