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. 2018 Feb 21;11(2):227–230. doi: 10.14202/vetworld.2018.227-230

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Copyright: © Abdou, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-1 — Agarose gel electrophoreses of polymerase chain reaction (PCR) products from multiplex PCR assays. Multiplex PCR assays were performed with a mixture of seven species-specific or group-specific primers for L. acidophilus, L. bulgaricus (same as L. delbrueckii subsp. bulgaricus), L. casei-group L. gasseri, L. plantarum, L. reuteri, and L. rhamnosus and two bacterial conserved primers. Lanes 1-12 designate the PCR product from each genomic DNA extracted from single or mixed cell suspension isolated from representative host used as PCR template. Lane 1: L. casei, L. gasseri; Lane 2: L. casei, L. acidophilus, L. delbrueckii; Lane 3: L. acidophilus, L. rhamnosus, L. plantarum, L. gasseri, L. delbrueckii; Lane 4: L. acidophilus; Lane 5: L. rhamnosus, L. plantarum, L. gasseri, L. delbrueckii; Lane 6: L. casei; Lane 7: Negative control; Lane 8: L. casei, L. acidophilus, L. delbrueckii; Lane 9: L. acidophilus, L. rhamnosus, L. gasseri, L. delbrueckii; Lane 10: L. acidophilus, L. rhamnosus, L. gasseri, L. delbrueckii; Lane 11: L. acidophilus, Lane 12: L. acidophilus, L. rhamnosus, L. plantarum; Lane M, 100 bp-DNA ladder.