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. 2018 Apr 9;25:33. doi: 10.1186/s12929-018-0433-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Detection of viral titers in mouse lung. Mice (n = 5/group) immunized with fusion proteins Flg-HA2–2-4M2ehs and FlgSh-HA2–2-4M2ehs were i.n. challenged with: A/Shanghai/2/2013(H7N9)-PR8-IDCDC (10LD₅₀); A/Aichi/2/68(H3N2) (10LD₅₀); A/California/07/09H1N1pdm09 (100MID); or А/Chicken/Kurgan/05/05RG(H5N1) (100MID). Viral titers were detected 6 days post challenge. The data are expressed as log TCID50. Horizontal bars indicate mean among 5 mice per group. The lower limit of detection is 0.5log TCID₅₀. Statistical significance was determined using the Mann-Whitney U-test. The P values between immunized and control group are indicated