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. 2018 Apr 10;13:54. doi: 10.1186/s13023-018-0798-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of DT on cytoplasmic lipid droplets accumulation in NCL patient NSCs. DT dose-dependently reduced the Nile red staining (a) in NCL NSCs. The quantitative analysis of Nile red fluorescence (b) revealed that clearance effect of lipid droplets ranged from 12.4% in the TPP1^E4/E6 NSCs to 34.9% in the TPP1^E4/IVS5 NSCs after the treatment with 20 μM δ-tocopherol. The images were taken with 40X objective lens. Data are displayed as mean ± SD. * P < 0.05, *** P < 0.001 compared to the untreated control