Fig. 3. Fluorescence micrographies in experiments carried out in HeLa cells using the in situ made copper(i) complexes: 75 μM CuSO4, 2 equiv. L4, 6 equiv. NaAsc. (A, B) Cells incubated with azide 1 (100 μM) and alkyne 2 (200 μM) for 1 h, followed by double washing with DMEM (2 × 5 min). (C, D) Cells after incubation with the copper containing mixture (30 min), DMEM washings (2 × 5 min), and treatment with 1 (100 μM) and 2 (200 μM) for 1 h, followed by double washing with DMEM (2 × 5 min). (E) Zoom of panel D. Basal levels of fluorescence were normalized by LUT equalization. Scale bar, 12.5 mm. (A and C and brightfield).
