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Measurement of cdh23-pcdh15 binding affinity by SPR. (A) Raw sensorgrams of binding between cdh23 at varying concentrations with immobilized pcdh15(N1). Inset plot shows peak response as a function of cdh23 concentration. Kd is 0.84 ± 0.03 μM for the canonical complex. (B) Sensorgrams for binding of cdh23 to pcdh15(N2) with a measured Kd of 5.12 ± 0.30 μM.
