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. Author manuscript; available in PMC: 2019 Mar 20.
Published in final edited form as: Biochemistry. 2018 Mar 6;57(11):1702–1710. doi: 10.1021/acs.biochem.7b01075

Table 2.

Summary of Binding Affinity Measurements of cdh23 and pcdh15 Complexes Using Different Experimental Methods and Conditions.

Method Temperature (°C) (μM)
cdh23 + pcdh15(N1)22 ITC 10 2.9 ± 0.4
cdh23 + pcdh15(N1) SPR 25 0.84 ± 0.03, n = 2
cdh23 + pcdh15(N2) SPR 25 5.12 ± 0.30, n = 2