Figure 7.

OA induces nuclear localization of TFEB in Ad-MAO-transduced cardiomyocytes. Immunofluorescence staining (red) with TFEB antibody in cardiomyocytes treated with TYR (500 μM) for 2 h or with TYR + OA added during the last 30 min (posttreatment). Nuclei are stained with DAPI (upper panel). Scale bar 20 μm. Quantification analysis of cells with nuclear localization of TFEB in percent of total nuclei (lower panel) (N = 3 independent experiments; for each experiment, 6 fields with about 15 cells were quantified per condition). ^∗ p < 0.05 versus CTRL and ^∗∗ p < 0.01 Tyr + OA versus TYR.