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. 2000 Mar;122(3):867–878. doi: 10.1104/pp.122.3.867

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Copyright © 2000, American Society of Plant Physiologists

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Distribution of XG-FucTase and UDPase in a membrane and soluble fraction. Golgi vesicles kept overnight in liquid nitrogen were thawed, centrifuged at 100,000g for 1 h, and the supernatant and pellet separated. XG-FucTase (A) and UDPase (B) activities were measured in the vesicles, supernatant (Sup.), and pellet. The total activity in the supernatant and pellet are compared with the total activity present in the vesicles before centrifugation. The data are the average activity for triplicate samples from one experiment out of two independent experiments with similar results.