Fig. 4. (a–e) U87MG tumor delineation and image-guided surgery at 12 h after the tail vein injection of CHS2 under 808 nm excitation (82 mW cm^–2), 1000 LP and 23 ms. (f) The tumor cells in this resected specimen were confirmed by high-power magnification (400×) of histological sections; (g) H&E-staining of the whole tumor. (h) The little muscle fibers marked as 1 were adjacent to the tumor tissue; (i) the brown band marked as 2 is the normal epithelium of the margin of the resected specimen; (j and k) the pink erythrocyte marked as 3 and 4 indicates the vessels in the margin of the tumor; (l) histological analysis of the excised para-cancerous tissue at 400× magnification. Cancer histologic characteristics were not detected in this para-cancerous tissue.