Figure 1.

Binding affinity of the ligand to SseK3. Isothermal titration calorimetry curves for the interaction of SseK3(14–333) and SseK3(14–335) with UDP-galactose (UDP-Gal), UDP-glucose (UDP-Glc), and UDP-glucosamine (UDP-GlcNAc) are shown. The experiments were executed in the presence of 5 mm MnCl₂. The integrated heat signals for the interaction were integrated as a function of the molar ratio of titrant to protein in the cell. The data were fitted to a 1:1 binding model, and the dissociation constants are reported for each experiment.