Table 1.
Thermodynamic quantities for the interaction of SseK3 constructs and UDP-glucose derivatives
| Protein/Ligands | Kd | ΔH | −TΔS | Sites |
|---|---|---|---|---|
| μm | kcal/mol | cal/mol degrees | ||
| SseK3(14–335)/no cation | NBDa | NBD | NBD | NBD |
| SseK3(14–335)/UDP-GlcNAc/Mg2+ | 10 ± 1.0 | −7.4 ± 0.4 | 0.7 ± 0.1 | 0.8 ± 0.1 |
| SseK3(14–335)/UDP-GlcNAc/Mn2+ | 1.9 ± 0.5 | −15.8 ± 0.3 | 8.1 ± 0.2 | 0.9 ± 0.1 |
| SseK3(14–335)/UDP-Gal/Mn2+ | 6.7 ± 0.8 | −8.9 ± 0.3 | 2.0 ± 0.4 | 0.8 ± 0.1 |
| SseK3(14–335)/UDP-Glu/Mn2+ | 2.6 ± 0.1 | −11.4 ± 0.8 | 4.0 ± 0.8 | 0.9 ± 0.1 |
| SseK3(14–333)/UDP-GlcNAc/Mn2+ | 6.3 ± 0.5 | −8.8 ± 0.5 | 1.9 ± 0.6 | 0.8 ± 0.1 |
| SseK3(14–333)/UDP-Gal/Mn2+ | 7.7 ± 1.6 | −7.1 ± 0.4 | 2.6 ± 0.5 | 0.8 ± 0.1 |
| SseK3(14–333)/UDP-Glu/Mn2+ | 5.6 ± 1.2 | −9.8 ± 0.5 | 2.8 ± 0.6 | 0.7 ± 0.1 |
a NBD, no binding detected.