Figure 10.

Altered post-PDT expression of NF-κB–regulated proteins other than iNOS: Effects of JQ1. U87 cells were sensitized with ALA-induced PpIX as described in the legends for Figs. 1 and 2. Immediately thereafter, DMSO vehicle control or 0.3 μm JQ1 was introduced, and cells were dark-incubated for 0, 3, 6, or 24 h after which they were recovered, solubilized, examined for total protein concentration, and then subjected to Western blot analysis for survivin (A), Bcl-xL (B), p21 (C), MMP-9 (D), and c-Myc (E). Western blotting is annotated as follows: non-irradiated control at 24 h, ALA; irradiated ALA-treated cells, ALA/hν; irradiated ALA-treated cells with JQ1, ALA/hν/JQ1. Effects of JQ1 without ALA/hν are also represented. The number below each band represents band intensity relative to β-actin and normalized to the ALA-only control. Each of the Western blot analyses shown is representative of two from duplicate experiments with nearly identical results. hν, light.