Figure 2.

Effect of JQ1 on iNOS up-regulation in U87 cells after a PDT challenge. A, cells were sensitized by ALA treatment and then either irradiated (1 J/cm²) or returned to the incubator for 24 h as a dark control (DC). Immediately after irradiation, cells either received DMSO vehicle alone or 0.3 μm JQ1 in DMSO and were recovered for iNOS and β-actin Western blot analysis after increasing periods of dark incubation, beginning at 0 h and extending to 24 h. hν, light. B, quantification of iNOS Western blotting bands expressed as mean fold changes ± S.E. relative to β-actin and normalized to the dark control without JQ1. The Western blotting shown in A is representative of three from replicate experiments with similar results. *, p < 0.01 versus vehicle control in each pair.