Figure 4.

Effect of FABP5 inhibition upon PGE₂ levels in activated THP-1 cells. A, Western blot of COX-2 induction in THP-1 cells after incubation with 5 μg/ml LPS for 2 or 24 h. B, PGE₂ levels in THP-1 cells before and after activation with LPS. The cells were incubated with vehicle (PBS; control) or 5 μg/ml LPS for 24 h to induce PGE₂ biosynthesis. The cells were subsequently washed and incubated with SBFI26, diclofenac, or vehicle (0.1% DMSO) for 2 h, and PGE₂ levels were quantified in the media. *, p < 0.05; **, p < 0.01 versus LPS-activated vehicle-treated cells (n = 6). C, effect of SBFI26 upon purified COX-1 and COX-2 activity (n = 4). D, PGE₂ biosynthesis in membrane fractions of control and LPS-activated THP-1 cells. Membranes were isolated 24 h after LPS stimulation and incubated with 1 μm arachidonic acid in the presence or absence of SBFI26 or diclofenac. **, p < 0.01 versus LPS-activated vehicle-treated membranes (n = 6). Error bars represent S.E.