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. 2018 Feb 22;293(14):5172–5184. doi: 10.1074/jbc.RA117.000246

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Figure 4. — Metabolic labeling of M. smegmatis strains overproducing EphD. The top panel shows a scheme of metabolic labeling of culture sets A, B, C, and D described under “Experimental procedures”; also shown is an autoradiogram of TLC analysis of radiolabeled fatty and mycolic acid methyl esters from whole cells of cultures A and B (radioactive label added 8 h before induction of recombinant EphD production) and C and D (label added in parallel with induction of recombinant EphD production). The samples were developed in n-hexane:ethyl acetate (95:5, v/v, three runs). α, α′, and epoxy indicate individual types of mycolic acid methyl esters; pHAM lane, M. smegmatis mc²155 pHAM; ephD_tb lane, M. smegmatis mc²155 pHAM-ephD_tb; ephD_smeg lane, M. smegmatis mc²155 pHAM-ephD_smeg.