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Metformin induces CD19-CAR T-cell apoptosis. (A) CD19-CAR T cells were treated with metformin (0, 1, 10, and 20 mM) for 24 hours. The apoptotic rate of CD19-CAR T cells was detected by flow cytometry. (B) Statistical analysis of the flow cytometry results. The results are represented as mean ± SD from three independent experiments. *P<0.05, **P<0.01 versus the control group.
Abbreviations: CAR, chimeric antigen receptor; Met, metformin; PI, propidium iodide.
