Figure 4.

Metformin increases the expression of p-AMPK and reduces the expression of p-mTOR and HIF1α inCD19-CAR T cells. (A) The CD19-CAR T cells were treated by 10 mM metformin for different times (0, 5, 10, 30, and 60 minutes), and the expression of p-AMPK, p-mTOR, and HIF1α was detected by Western blotting analysis. (B) Statistical analysis of relative p-AMPK, p-mTOR, and HIF1α protein expression results. *P<0.05, **P<0.01 versus the control group. Data are represented as mean ± SD from three independent experiments.

Abbreviations: CAR, chimeric antigen receptor; p-AMPK, phosphor AMP-activated protein kinase.