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. 2018 Apr 3;11:649–659. doi: 10.2147/JPR.S153544

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© 2018 Liu et al. This work is published and licensed by Dove Medical Press Limited

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Expression and activation of Shh signaling after chronic morphine treatment.

Notes: (A) Examples of Western blot analysis and data summary (n=6 in each group) showing a time-dependent increased expression of Shh, Ptch1, and Smo in the SC of the mice. (B) Examples of Western blot analysis and data summary (n=6 in each group) showing a time-dependent increased expression of Gli1 in nuclear extract of the SC of the mice. (C) Immunofluorescence staining shows the expression and location of Shh in the dorsal horn of the SC (n=4 in each group) (bar: 50 μm). (D) Examples of Western blot analysis and data summary showing a time-dependent increased expression of Shh and Gli1 in DRG (n=6 in each group). (E) Immunofluorescence staining shows the expression of Shh in DRG (n=6 in each group) (bar: 250 μm). (F) ELISA showing the concentration of Shh in DRG and SC (n=6 in each group). For immunofluorescence staining, samples were collected on day 7 after the first morphine injection. *P<0.05, **P<0.01 versus sham group.

Abbreviations: SC, spinal cord; DRG, dorsal root ganglion; i.t., intrathecally; Mor, morphine; ELISA, enzyme linked immunosorbent assay.