Figure 1. Experimental protocol to evaluate chemopreventive activity in F344 rats. Rats were divided into two control (untreated and treated) groups and four experimental groups. The rats were fed a control diet (AIN-76A) or diet supplemented with different doses of Capsosiphon fulvescens (1 or 2%) and Hizikia fusiforme (2 or 6%), for the 11-week experimental period. Rats in groups 2-6 received subcutaneous injections of azoxymethane (AOM; 15 mg/kg body weight) once a week for 2 weeks. Group 1 served as an untreated control (1 ml saline/kg by subcutaneous injection + control diet) and group 2 served as the treated control (15 mg AOM/kg body weight by subcutaneous injection + control diet).