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. 2000 Mar;122(3):907–914. doi: 10.1104/pp.122.3.907

Figure 5.

Figure 5

Suppression of a S. cerevisiae his1 null mutant, BY1001, with the Arabidopsis AtATP-PRT cDNAs. A, Construction of a his1::LEU2 null allele on chromosome 5 (Chr.V). Restriction enzyme sites are designated: B, BamHI; Bg, BglII; Sa, SalI; and Xh, XhoI. B, Growth of a S. cerevisiae his1 mutant (BY1001). Strain BY1001 was transformed with either pYES2 (empty plasmid), pKF110 (plasmid carrying S. cerevisiae HIS1 coding region), pKF251 (plasmid harboring AtATP-PRT1 cDNA truncated at the chloroplast transit peptide portion), or pKF252 (plasmid containing AtATP-PRT2 cDNA without the chloroplast transit peptide region). After the transformation, the cells were cultivated on a minimal-Gal plate supplemented with an amino acids mixture without l-His, Leu, and Ura (SC/Gal-His- Leu-Ura).