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. 2018 Jan 4;32(3):1403–1416. doi: 10.1096/fj.201700300R

Figure 1.

Figure 1.

A, B) Overexpression of CerS2 results in cardiomyocyte mitochondrial dysfunction and apoptosis. AC16 cardiomyocytes ovexpressing CerS2 exhibited reduced basal oxygen consumption, ATP turnover, and respiration capacity. C, D) Decreased mitochondrial membrane potential was detected with MitoTracker green (mitochondria) and DAPI (nuclei) in cells with overexpressed CerS2. E, F) Cardiomyocytes overexpressing CerS2 showed increased Annexin V detection after 24 and 48 h transfection: Annexin V, green; DAPI, blue (E) and quantification (F). G, H) Cells overexpressing CerS2 showed increased cleavage of caspase 3 after 24 and 48 h. I) An increase in LC ceramides in the mitochondrial enriched fraction was observed in cells overexpressing CerS2 for 48 h. Student’s t test (n = 3–5). Scale bars, 40 µm. *P < 0.05, **P < 0.01, ***P < 0.001 compared with control (ctrl).