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Inhibition of autophagy enhances PI3K inhibitor–induced apoptosis. Cells were transfected with siRNA targeting ATG5 (A), ATG7 (B), or nonsilencing control; 2 d after transfection, cells were treated ± 1 μM GDC-0941. Lysates were collected after 24 h of drug treatment and analyzed by immunoblotting using the indicated antibodies. FL, full-length; CL, cleaved.
