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Bim and Puma are elevated by PI3K inhibition and CQ treatment. Cells were pretreated ± 1 μM fulv for 24 h, then treated without or with 1 μM GDC-0941, 50 μM CQ, or 1 μM fulv for 24 h. Lysates were analyzed by immunoblot using the indicated antibodies. EL, extra-long; L, long; S, short.
