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. 2017 Nov 16;32(3):1196–1206. doi: 10.1096/fj.201700518R

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This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (http://creativecommons.org/licenses/by-nc/4.0/) which permits noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Adult reduction of Ins2 gene dose reverses obesity in mice fed an HFD, primarily because of reduced mass of gonadal and perirenal fat pads. A) Percentage change in mass of control littermate (n = 39) and experimental (n = 29) mice 5 wk after tamoxifen injection. B) Inguinal, gonadal, perirenal, and mesenteric WAT depots and BAT from mice were weighed after 16 h without food and again at 15 min after an insulin stimulation (n = 16, 11). C) Representative transsectional images obtained by micro-CT (left) and quantification (right) of percentage change in subcutaneous and visceral fat depots after tamoxifen injection (n = 14, 17). D) Daily food intake was measured for 2 wk before, and 4 wk after, tamoxifen injection (n = 14, 19; control n is listed first throughout). Arrow: the week of tamoxifen injection. E) Representative sections of gonadal (n = 7, 10) and perirenal (n = 8, 11) adipose tissue (top; hematoxylin and eosin staining) and analysis of adipocyte size distribution (bottom; perilipin staining). F–H) NEFA (F), free glycerol (G), and glyceride (H) levels (n = 6, 8) were measured after food was withheld for 16 h and again 15 min after insulin stimulation. Unless otherwise indicated, measurements were conducted in samples collected from mice between 5 and 7 wk after tamoxifen injection. Data are means ± sem. *P < 0.05.