Figure 2.

Inhibition of L1 inhibition by ethanol and methanol in L1 constructs bearing leucine mutations of 4 OLIPS. NIH/3T3 cells were transiently transfected with hL1 in which residues were replaced with leucine or alanine, as indicated. Cells were harvested for cell aggregation assays performed in the absence and presence of 100 mM ethanol or 100 mM methanol. Alcohol inhibition of adhesion in mutant constructs was normalized to values obtained in L1-WT. Shown are means ± sem levels of alcohol inhibition from 9 to 142 independent experiments (F = 9.87; P < 0.0001). *P < 0.05; **P < 0.01; ***P < 0.001 comparing inhibition in mutants with WT or comparing ethanol with methanol (brackets).