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. 2017 Nov 8;32(3):1364–1374. doi: 10.1096/fj.201700970

Figure 4.

Figure 4.

Effect of OLIPS mutations on L1 association with ankyrin and L1 sensitivity to ethanol. A). Representative Western blot and densitometric analysis of L1 association with ankyrin-G, spectrin, and actin in cells expressing single OLIPS leucine mutations in the absence and presence of the Y1229F mutation (F = 7.72; P < 0.0001). *P < 0.05; **P < 0.01; ***P < 0.001 (n = 6–17). B) Ethanol inhibition of L1 adhesion in the constructs shown in A (F = 9.27; P < 0.0001). *P < 0.05; **P < 0.01; ***P < 0.001 (n = 9–142). C) Association of ankyrin-G, spectrin, and actin with L1 in 2A2-L1s cells vs. 3C3-L1i cells (F = 10.69; P < 0.0001). **P < 0.01 (n = 6–11).