Decreased mitochondrial ceramides in S135F mutant HT-22 cells. Mitochondrial (Mito.; A), cytoplasmic (Cyto.; B), and nuclear (Nuc.; C) fractions of HT-22 cells were isolated and used for lipid measurements with LC/MS. Mitochondrial fractions demonstrate reduced total ceramide (*P < 0.05, n = 4) and cytoplasmic fractions with increased total ceramide (**P < 0.01, n = 4), and there is no change in nuclear ceramides (P = 0.95, n = 4).