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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Dev Dyn. 2018 Jan 18;247(4):660–671. doi: 10.1002/dvdy.24615

Figure 5. Knockdown of zygotic Mov10 causes decreases in eye and body size.

Figure 5

A) Whole-mount images of control morpholino (Con) injected tadpoles at stage 36. A total of 46 tadpoles were analyzed, all of which exhibited this normal phenotype. B) Whole-mount images of zygotic Mov10 knockdown (z-MO) tadpoles at stage 36. A total of 48 tadpoles were analyzed and 45 of them showed a small eye phenotype. (C, D) Fluorescein images of the tadpoles from A and B, respectively, showing the distribution of the fluorescein-tagged morpholinos. E) RT-PCR using Mov10 primers for control and z-MO injected embryo cDNA showing effective splicing blocking and absence of Mov10 splice junction (PCR product expected size is 197nt, + reaction included cDNA, - reactions did not include cDNA). As a positive control, ODC (Ornithine Decarboxylase) was found to be present in both samples (PCR product expected size is 221nt). F) Graph showing eye diameter in μm from control morpholino (con) injected and z-MO injected embryos measured at stage 36. A total of 67 tadpoles were analyzed. G) Graph showing overall anterior-posterior (AP) length in mm from control morpholino (con) injected and z-MO injected embryos measured at stage 36. A total of 67 tadpoles were analyzed. Error bars represent SEM, **p<0.01 (Student’s t-test, two-tailed). Scale bar in D equals 800μm.