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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Gastroenterology. 2017 Dec 24;154(5):1449–1464.e20. doi: 10.1053/j.gastro.2017.12.019

Figure 3. NR1D1 regulates IL18 and IL1B maturation and secretion in a NLRP3 inflammasome-dependent manner.

Figure 3

Caspase-1 protein (sub-unit p10, p20, p35, p45) expression in supernatant (A) or lysate (B) from LPS-primed (LPS) and ATP-activated (LPS/ATP) or not (/) BMDMs from Nr1d1+/+ (Rα+/+) and Nr1d1−/− (Rα−/−) mice (representative of 3 independent experiments). (C) IL1B and (D) IL18 secreted by LPS-primed and ATP-activated BMDMs isolated from Nr1d1+/+ or Nr1d1−/− mice that were treated with low dose of caspase inhibitor z-VAD-fmk (z-VAD-fmk) or not (Vehicle) (n=3). (E) IL1B secreted by LPS-primed and ATP activated (ATP) or not (CTRL) BMDMs treated with (hemin) or its vehicle (vehicle) and with NLRP3 inhibitor MCC950 or not (Saline) (n=3). (F) IL18 secreted by LPS-primed and ATP-activated (ATP) or not (CTRL) MDMs treated with (hemin) or its vehicle (vehicle) and with NLRP3 inhibitor MCC950 or not (Saline) (n=3). (G) IL1B and (H) IL18 secreted by LPS-primed and ATP-activated BMDMs isolated from Nr1d1+/+ or Nr1d1−/− mice in which NLRP3 was silenced (siRNA NLRP3) or not (siRNA CTRL) (n=3). The top western blot (G) shows NLRP3 protein silencing. Data are represented as means ± SD. ***p<0.001 as determined by two-way ANOVA followed by a Bonferroni post-hoc test.